High LET Molecular Radiation Biology

Works are being carried out to study the response of cancer and normal cell lines exposed to high LET radiation.

  • Study on the role of PARP-1 in DNA double strand breaks repair and apoptosis in cancer cell lines by 12C6+ ion beam irradiation.

  • Study on genotoxic effect and mRNA expression of human cord blood stem cells and blood lymphocytes on exposure to high LET radiation. Research on investigation of mechanism of neoplastic transformation in normal cells and radiation induced leukemogenesis in hematopoietic stem cells is also carried out.

  • Study on activation of sensor proteins such as ATM/ATR and kinases involved in DNA repair, cell cycle arrest and apoptosis pathway in lung cancer cells irradiated with high LET radiation.

  • Evaluation of factors responsible for Bystander effect of high LET exposed cancer cells.

  • Assessment of High and Low Let radiation effects on Chinese hamster ovary cells(CHO) or Murine Macrophage cells with exploration of cellular mechanism involved. The objective is to investigate the time-dependent regulation by Moringa oleifera leaf extract and its active components (Quercetin, Ferulic Acid, Rutin) on the activity of the main transcription factors (NF-κB and nuclear transcription factor erythroid 2p45-related factor (Nrf2)) related to antioxidant defense in radiation induced oxidative stress pathways.